Research Equipment Center
Igor M. Konovets
Ph.D.Address: 12 Geroyiv Stalingrada prosp., Kyiv. Lab. No 216.
Tel: 38 (044) 254-11-77, 254-11-53.
Fax: 38 (044) 418-22-32
E-mail This email address is being protected from spambots. You need JavaScript enabled to view it.
- Hydroecological Analytical Centre (center for associated use of scientific instruments/equipment by employees of the National Academy of Science of Ukraine) is organized for the purpose of rational use of unique and valuable instruments/equipment of domestic or importable manufacturing.
Light microscope for scientific and routine tasks “Axio Imager A1” (Carl Zeiss)
Usage: Axio Imager A1 – a microscope for general and applied use in the field of biology and medicine. Microscope satisfies modern scientific and technical requirements and international standard ISO 9000.
Methods: using transmitted light – brightfield and differential interference contrast; using reflected light – epi-fluorescence. Currently available objectives on the system are 2.5×/0.085 5×/0.15, 10×/0.30, 20×/0.50, 40×/0.75, 63×/0.85 and 100×/1.30.
For operation with differential interference contrast DIC slider EС PN 5× 0, 10× І, 20× ІІ, РА 20× ІІ and DIC slider EС PN 40× ІІ, EС PN 100× ІІІ for objectives 10×/0,30, 20×/0,50, 40×/0,75 and 100×/1,30 are available.
Epi-fluorescence illumination system provides Köhler principle (field and aperture diaphragm in reflected light) with 100 Wt mercury light source.
For operation with epi-fluorescence microscopy, several sets of filter systems are available:
- set No 15 (excitation ВР 546/12, beamsplitter FT 580, emission LP 590);
- set No 44 (excitation ВР 475/40, beamsplitter FT 500, emission LP 530/50);
- set No 49 (excitation G 365, beamsplitter FT 395, emission BP 445/50).
Usage: quantitative and qualitative determination of polar nonvolatile thermostable and thermolabile organic substances (pharmaceuticals, biologically active substances, water-soluble pesticides, PAHs etc. in the matrixes of different difficulty.
Detectors: HPLC is equipped with diode-array detector (190–950 nm), fluorescent detector and single-quadruple mass detector (API and APCI ion sources, m/z 80–3000 ±0.13 u, 2500–5250 u per second).
Light microscope for scientific and routine tasks “Axio Imager A1” (Carl Zeiss
Application of cytogenetic methods in biotesting. Investigation of mitotic cycle of the apical meristem cells of roots of plant test-objects (photo by Kipnis L. and Goncharova M.).
Studying of functional activity of cells. Staining of active nucleolus-forming regions of chromosomes with silver nitrate (photo Stoyka Yu.)
Investigation of phytoplankton species composition. Diatoms Melosira varians Ag.; Diatoma vulgare Bory and Navicula tripunctata (O. F. Müll.) Bory (photo by Semenyuk N.)
Studying of morphological parameters of microalgae using Axio Vision 4 software. Green alga Volvox polychlamys Korshikov, coenobium and individual cell (photo by Yarmoshenko L.)
Parasite Aspidogaster conchicola: A – brightfield; B – differential interference contrast (photo by Yuryshynets V.)
Parasite Aspidogaster conchicola: A – brightfield; B – differential interference contrast (photo by Yuryshynets V.)
Studying of bacterial plankton. Dyes: A – DAPI; B – Live/dead Baclight viability kit (SYTO 9® and PI); C – CTC (photo by Starosila Ye.)
HPLC System “Agilent 1200” SL/DAD/FD/MSD 6130
Applicability of chromatography methods in dependence to target substance properties.
Applicability of HPLC-MS ionization sources in dependence to target substance polarity and molecular weight.
Quantification of S-adenosylmethionine and S-adenosylhomocysteine in human placenta and placental explants under homocysteine treatment / R. Rodriguez, O. Vakulenko, S. Ralchenko, A. Kostiuk, L. Porublyova, I. Konovets, I. Voronina, M. Obolenskaya // International Journal of Mass Spectrometry, 2017, ISSN 1387–3806, http://dx.doi.org/10.1016/j.ijms.2017.08.002.
Features of energy supply of fish adaptation to fipronil and dimethoate (in Ukrainian) / M.О. Savluchynska, I.M. Konovets, O.M. Arsan, M.G. Mardarevych (Особливості енергозабезпечення процесів адаптації риб до різних концентрацій фіпронілу та диметоату у водному середовищі / М.О. Савлучинська, І.М. Коновець, О.М. Арсан, М.Г. Мардаревич // Наук. зап. Терноп. нац. пед. ун-ту. Сер. Біол., 2017, № 2 (69) С. 70–75).
Analysis of phenolcarbonic acids content in phytomass of higher aquatic plants / O.M. Usenko, I.N. Konovets // Hydrobiological Journal Vol. 50, Issue 5, 2014, P. 47–60.
Patent for invention №109838, Ukraine. Method for determination of the qualitative and quantitative composition of phenolcarbonic acids in water using HPLC/MS (in Ukrainian) / O.M. Usenko, I.M. Konovets. Патент на винахід №109838. Спосіб визначення якісного і кількісного складу фенолкарбонових кислот у воді за допомогою хромато-мас-спектрофотометра / О.М. Усенко, І.М. Коновець; заявник та патентовласник Інститут гідробіології НАН України; опубл. 12.10.2015 р. Бюл. №19.
Patent for invention №103091, Ukraine. Method for determination of the qualitative composition of phenolcarbonic acids in the biomass of higher aquatic plants using HPLC/MS (in Ukrainian) / O.M. Usenko, I.M. Konovets (Патент на винахід №103091, Україна. Спосіб визначення якісного складу фенолкарбонових кислот у біомасі вищих водяних рослин за допомогою хромато-мас-спектрофотометра / О.М. Усенко, І.М. Коновець; заявник та патентовласник Інститут гідробіології НАН України; опубл. 10.09.2013, Бюл. №17).